Kinetic characterization of a crude β-d-glucosidase from Aspergillus wentii Pt 2804

@inproceedings{Srivastava1984KineticCO,
  title={Kinetic characterization of a crude β-d-glucosidase from Aspergillus wentii Pt 2804},
  author={Smita Srivastava and Kalpathi S. Gopalkrishnan and K. B. Ramachandran},
  year={1984}
}
Abstract The kinetic characteristics of β- d -glucosidase (cellobiase, β- d -glucosidase glucohydrolase, EC 3.2.1.21) from the filtered broth of a well grown culture of Aspergillus wentii have been studied. Both cellobiose and 4-nitrophenyl-β- d -glucoside (4NPG) were used as substrates and values of K m , V max for both the substrates were determined. Activity was maximum over a pH range of 4.5–5.5 but declined sharply beyond 5.5 for both substrates. The optimum temperature was between 60 and… CONTINUE READING