Kinetic characterization of N-acetyl-D-glucosamine kinase from rat liver and kidney.

@article{Allen1980KineticCO,
  title={Kinetic characterization of N-acetyl-D-glucosamine kinase from rat liver and kidney.},
  author={M. B. Allen and D. G. Walker},
  journal={The Biochemical journal},
  year={1980},
  volume={185 3},
  pages={
          577-82
        }
}
1. Under normal assay conditions the N-acetyl-D-glucosamine kinases from rat liver and kidney show a pH-dependent lag phase before reaching a steady state, which is probably due to reversible dissociation of the dimeric enzyme. 2. The enzyme catalyses the phosphorylation of N-acetyl-D-glucosamine, N-acetyl-D-mannosamine and D-glucose at pH 7.5, with apparent Km values of 0.06, 0.95 and 600 mM respectively for the enzyme from liver and 0.04, 1.0 and 410 mM respectively for the kidney enzyme. It… Expand
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