Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures
@article{Hertz2004KineticCO, title={Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures}, author={Leif Hertz and Arne Schousboe and Norma Boechler and Srimathie Mukerji and Sergey Fedoroff}, journal={Neurochemical Research}, year={2004}, volume={3}, pages={1-14} }
Kinetics for uptake and release of glutamate were measured in normal, i.e., nontransformed, astrocytes in cultures obtained from the dissociated, cortexenriched superficial parts of the brain hemispheres of newborn DBA mice. The uptake kinetics indicated a minor, unsaturable component together with an intense uptake following Michaelis-Menten kinetics. TheKm (50 μM) was reasonably comparable to the corresponding values in brain slices and in other glial preparations. TheVmax (58.8 nmol min−1 mg…
78 Citations
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References
SHOWING 1-10 OF 66 REFERENCES
Uptake and metabolism of GABA in astrocytes cultured from dissociated mouse brain hemispheres
- Biology, ChemistryNeurochemical Research
- 2004
During three weeks of culturing the activity of the transaminase rose from a low neonatal value toward the level in the adult brain, and the uptake kinetics indicated an unsaturable component together with an uptake following Michaelis-Menten kinetics.
UPTAKE AND METABOLISM OF GLUTAMATE IN ASTROCYTES CULTURED FROM DISSOCIATED MOUSE BRAIN HEMISPHERES
- Biology, ChemistryJournal of neurochemistry
- 1977
The results suggest that glia cells are important for the uptake of glutamate from synaptic clefts and for the subsequent metabolism of glutamate.
UPTAKE AND METABOLISM OF GLUTAMATE BY ISOLATED TOAD BRAINS CONTAINING DIFFERENT LEVELS OF ENDOGENOUS AMINO ACIDS
- BiologyJournal of neurochemistry
- 1975
In both the FWA and HOA toad brain systems some [U‐14C]glutamate was metabolized to aspartate and GABA; in both systems the specific radioactivity (SA) of glutamine in the tissue was from two to four times greater than that of glutamate; also the SA of glutamines released into the medium was higher by several orders of magnitude than the SA in brain tissues.
POSTNATAL ALTERATIONS IN EFFECTS OF POTASSIUM ON UPTAKE AND RELEASE OF GLUTAMATE AND GABA IN RAT BRAIN CORTEX SLICES
- BiologyJournal of neurochemistry
- 1976
The results are compatible with the existence of different transport systems for the two amino acids, and suggest that glutamate may exert other functions in addition to its role as a putative transmitter.
Structural requirements for the inhibition for L-glutamate uptake by glia and nerve endings
- BiologyBrain Research
- 1975
Amino acid transport in isolated neurons and glia.
- Biology, ChemistryAdvances in experimental medicine and biology
- 1976
It is believed that work along these lines tends to firmly establish a direct role for glial cells as modulators of neuronal excitability and represents a convergence between transmitter amino acid neuropharmacology and cellular biochemistry.
[14C]GLUTAMATE UPTAKE AND COMPARTMENTATION IN GLIA OF RAT DORSAL SENSORY GANGLION
- Biology, ChemistryJournal of neurochemistry
- 1974
The characteristics of the uptake of l‐[U‐14C] glutamate into rat dorsal sensory ganglia were investigated and the results are discussed in relation to current knowledge of metabolic compartmentation in nervous tissue.
High content of glutamate and of ATP in Astrocytes cultured from rat brain hemispheres: effect of serum withdrawal and of cyclic AMP
- BiologyJournal of neurochemistry
- 1975
The purpose of the present paper has been to study the glutamate content in astrocyte cultures from neonatal rat brain and it has been shown that the concentration of glutamine in the cultivation medium affects the growth of the C6 glioma cell.
HIGH AFFINITY UPTAKE OF L‐GLUTAMATE AND L‐ASPARTATE BY GLIAL CELLS
- BiologyJournal of neurochemistry
- 1977
The presence of an efficient, high affinity uptake system specific for L‐glutamate and L‐aspartate has been demonstrated in cultured glial cells originating from syrian hamster astroblasts and the temperature dependence and the susceptibility to metabolic inhibitors suggest that the uptake is mediated by an active transport system.