Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures

Leif Hertz, Arne Schousboe, Norma Boechler, Srimathie Mukerji, Sergey Fedoroff
Published 1978 in Neurochemical Research

Abstract

Kinetics for uptake and release of glutamate were measured in normal, i.e., nontransformed, astrocytes in cultures obtained from the dissociated, cortexenriched superficial parts of the brain hemispheres of newborn DBA mice. The uptake kinetics indicated a minor, unsaturable component together with an intense uptake following Michaelis-Menten kinetics. TheK… (More)

DOI: 10.1007/BF00964356

A. SCHOUSBOE, E. BOOK, L. HERTZ
Page 435,
1977

L. HERTZ, A. SCHOUSBOE, S. FEDOROFE
Trans. Am. Soc. Neurochem
1977

Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures

Abstract

Topics

5 Figures and Tables

Cited By

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Slides referencing similar topics

Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures

Abstract

Topics

5 Figures and Tables

Cited By

Bi-phasic regulation of glycogen content in astrocytes via Cav-1/PTEN/PI3K/AKT/GSK-3β pathway by fluoxetine

Effect of monensin on deoxyglucose uptake in cultured astrocytes: energy metabolism is coupled to sodium entry.

Changes in routine laboratory tests and survival in amyotrophic lateral sclerosis

Glutamine-Glutamate Cycle Flux Is Similar in Cultured Astrocytes and Brain and Both Glutamate Production and Oxidation Are Mainly Catalyzed by Aspartate Aminotransferase

Maxi-anion channels play a key role in glutamate-induced ATP release from mouse astrocytes in primary culture.

Severe Convulsions and Dysmyelination in Both Jimpy and Cx32/47 −/− Mice may Associate Astrocytic L-Channel Function with Myelination and Oligodendrocytic Connexins with Internodal Kv Channels

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Glucose replaces glutamate as energy substrate to fuel glutamate uptake in glutamate dehydrogenase-deficient astrocytes.

Ammonium Increases Ca2+ Signalling and Up-Regulates Expression of TRPC1 Gene in Astrocytes in Primary Cultures and in the In Vivo Brain

Methodological Limitations in Determining Astrocytic Gene Expression

References

Effect of dBcAMP and serum withdrawal on morphological and biochemical differentiation of normal astrocytes in cultures

Glutamate uptake, metabolism and release in cultured normal astrocytes

Glutamic acid and excitatory nerve endings: reduction of glutamic acid uptake after axotomy.

High affinity uptake of L-glutamate and L-aspartate by glial cells.

Uptake and metabolism of GABA in astrocytes cultured from dissociated mouse brain hemispheres

Uptake and metabolism of glutamate in astrocytes cultured from dissociated mouse brain hemispheres.

Amino acid transport in isolated neurons and glia.

Amino acid transport in spinal and sympathetic ganglia.

Cerebral uptakes and exchange diffusion in vitro of L- and D-glutamates.

Comparison of skeletal muscle monolayer cultures initiated with cells dissociated by the vortex and trypsin methods

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