Kinetic analysis of wild-type and YMDD mutant hepatitis B virus polymerases and effects of deoxyribonucleotide concentrations on polymerase activity.

@article{Gaillard2002KineticAO,
  title={Kinetic analysis of wild-type and YMDD mutant hepatitis B virus polymerases and effects of deoxyribonucleotide concentrations on polymerase activity.},
  author={Richard K Gaillard and Jennifer Barnard and Vincent A Lopez and Paula Hodges and Eric T Bourne and Lance D. Johnson and Marchelle I Allen and Patrick Condreay and Wayne H. Miller and Lynn D. Condreay},
  journal={Antimicrobial agents and chemotherapy},
  year={2002},
  volume={46 4},
  pages={1005-13}
}
Mutations in the YMDD motif of the hepatitis B virus (HBV) DNA polymerase result in reduced susceptibility of HBV to inhibition by lamivudine, at a cost in replication fitness. The mechanisms underlying the effects of YMDD mutations on replication fitness were investigated using both a cell-based viral replication system and an in vitro enzyme assay to examine wild-type (wt) and YMDD-mutant polymerases. We calculated the affinities of wt and YMDD-mutant polymerases for each natural… CONTINUE READING

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In vivo dynamics and pathogenicity of wild-type and resistant Hepatitis B virus during long-term lamivudine monotherapy - a clinical note.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology • 2000
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