Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase.

@article{Carvalho2006KineticAO,
  title={Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase.},
  author={Luiz Pedro S de Carvalho and John S Blanchard},
  journal={Archives of biochemistry and biophysics},
  year={2006},
  volume={451 2},
  pages={141-8}
}
Mycobacterium tuberculosis alpha-isopropylmalate synthase (MtIPMS) is a member of the family of enzymes that catalyze a Claisen-type condensation. In this work we characterized the monovalent and divalent specificity of MtIPMS using steady-state kinetics. The monovalent cation dependence of the kinetic parameters of substrates and divalent metals indicates… CONTINUE READING