Kinetic analysis of the RNAi enzyme complex

@article{Haley2004KineticAO,
  title={Kinetic analysis of the RNAi enzyme complex},
  author={Benjamin Haley and Phillip D. Zamore},
  journal={Nature Structural \&Molecular Biology},
  year={2004},
  volume={11},
  pages={599-606}
}
  • B. Haley, P. Zamore
  • Published 30 May 2004
  • Biology, Medicine
  • Nature Structural &Molecular Biology
The siRNA-directed ribonucleoprotein complex, RISC, catalyzes target RNA cleavage in the RNA interference pathway. Here, we show that siRNA-programmed RISC is a classical Michaelis-Menten enzyme in the presence of ATP. In the absence of ATP, the rate of multiple rounds of catalysis is limited by release of the cleaved products from the enzyme. Kinetic analysis suggests that different regions of the siRNA play distinct roles in the cycle of target recognition, cleavage, and product release… Expand
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