Kinetic analysis of glutamate-induced chloride current in Aplysia neurones: a 'concentration clamp' study.

@article{Ikemoto1988KineticAO,
  title={Kinetic analysis of glutamate-induced chloride current in Aplysia neurones: a 'concentration clamp' study.},
  author={Y. Ikemoto and Norio Akaike and Kyoichi Ono},
  journal={European journal of pharmacology},
  year={1988},
  volume={150 3},
  pages={303-11}
}
L-Glutamate (Glu) applied by the 'concentration clamp' technique to isolated neurones of Aplysia induced a chloride current (ICl) by activating a single population of the channel. The concentration-response curve for the peak ICl gave a dissociation constant of 1.3 x 10(-4) M and a Hill coefficient of 1.8. The current-voltage relationship was linear in the voltage range examined (-60 to +10 mV). The activation phase of the ICl followed a single-exponential time course and desensitization was… CONTINUE READING