Kinetic analysis of four HIV-1 reverse transcriptase enzymes mutated in the primer grip region of p66. Implications for DNA synthesis and dimerization.

Abstract

The highly conserved primer grip region in the p66 subunit of HIV-1 reverse transcriptase (RT) is formed by the beta12-beta13 hairpin (residues 227-235). It has been proposed to play a role in aligning the 3'-OH end of the primer in a position for nucleophilic attack on an incoming dNTP. To analyze the importance of the primer grip for RT function, mutant… (More)

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Cite this paper

@article{Whrl1997KineticAO, title={Kinetic analysis of four HIV-1 reverse transcriptase enzymes mutated in the primer grip region of p66. Implications for DNA synthesis and dimerization.}, author={Birgitta M. W{\"{o}hrl and Rainer Krebs and Sara H Thrall and Stuart F J Le Grice and Axel J. Scheidig and Roger S. Goody}, journal={The Journal of biological chemistry}, year={1997}, volume={272 28}, pages={17581-7} }