Kinetic analysis of estrogen receptor/ligand interactions.

  title={Kinetic analysis of estrogen receptor/ligand interactions.},
  author={Rebecca L. Rich and Lise R. Hoth and Kieran F. Geoghegan and Thomas A. Brown and Peter K Lemotte and Samuel P Simons and Preston Hensley and David G. Myszka},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={99 13},
Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the association and dissociation phase binding responses were shown to be reproducible and could be fit globally to a simple interaction model to extract reaction rate constants. On average, antagonist… CONTINUE READING


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