Kinetic analysis of cardiolipin synthase: A membrane enzyme with two glycerophospholipid substrates

Mitochondrial cardiolipin synthase catalyzes the transfer of a phosphatidyl moiety from phosphatidyl-CMP PtdCMP) to phosphatidylglycerol (PtdGro) in the presence of specific divalent cations. The synthase was solubilized fromSaccharomyces cerevisiae mitochondria and purified about 300-fold. The partially purified enzyme was part of a medium-size, mixed… CONTINUE READING