Kinetic analysis and modelling of the allosteric behaviour of liver and muscle glycogen phosphorylases

@article{CuadriTom2006KineticAA,
  title={Kinetic analysis and modelling of the allosteric behaviour of liver and muscle glycogen phosphorylases},
  author={Celia Cuadri-Tom{\'e} and Carmen Bar{\'o}n and Vicente Jara-P{\'e}rez and Antonio Parody-Morreale and Jose C. Mart{\'i}nez and Ana C{\'a}mara-Artigas},
  journal={Journal of Molecular Recognition},
  year={2006},
  volume={19}
}
Allosteric enzymes have very complex kinetic behaviours which are primarily interpreted through simplified models. To describe the functional properties of liver and muscle glycogen phosphorylase isozymes we have developed an experimental strategy based on the measurements of initial reaction rates in the presence of different concentrations of the effectors glucose‐1‐phosphate and methyl‐xanthines. Using the extensive structural information available for the two glycogen phosphorylase… 
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Investigations on the mechanism of allosteric activation of rabbit muscle glycogen phosphorylase b by AMP
TLDR
This study has employed linkage analysis in combination with structural perturbations via site-directed mutagenesis to test kinetic models of activation of phosphorylase b by AMP, and to examine the roles of the N-terminus, the acidic patch, α-helix 1 and the 280’s loop in activation byAMP.
Effects of phosphorylation on the activity of glycogen phosphorylase in mutton during incubation at 4 °C in vitro.
Glutathione-dependent reduction of arsenate by glycogen phosphorylase responsiveness to endogenous and xenobiotic inhibitors.
  • Z. Gregus, B. Németi
  • Biology, Chemistry
    Toxicological sciences : an official journal of the Society of Toxicology
  • 2007
TLDR
It was shown that a GPa-rich extract of rat liver contained GSH-dependent As(V)-reducing activity that was inhibited by specific GPIs, suggesting that the liver-type GPa can also catalyze reduction of As( V), supporting that the latter is coupled to glycogenolysis.
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
TLDR
The results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HDS1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus.
Statistical mechanics of Monod-Wyman-Changeux (MWC) models.
Isothermal Titration Calorimetry: Thermodynamic Analysis of the Binding Thermograms of Molecular Recognition Events by Using Equilibrium Models
© 2013 Martinez et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which…
Effects of different ATP contents on phosphorylation level of glycogen phosphorylase and its activity in lamb during incubation at 4 ℃ in vitro

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