Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

  title={Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions},
  author={Russell Higuchi and Carita Fockler and Gavin Dollinger and Robert Malcolm Watson},
We describe a simple, quantitative assay for any amplifiable DNA sequence that uses a video camera to monitor multiple polymerase chain reactions (PCRs) simultaneously over the course of thermocycling. The video camera detects the accumulation of double-stranded DNA (dsDNA) in each PCR using the increase in the fluorescence of ethidium bromide (EtBr) that results from its binding duplex DNA. The kinetics of fluorescence accumulation during thermocycling are directly related to the starting… 

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