• Corpus ID: 1132442

Kinetic Modelling and Inference of Hyperpolarized 13C Molecules in Cancer Metabolism

@article{Zhao2017KineticMA,
  title={Kinetic Modelling and Inference of Hyperpolarized 13C Molecules in Cancer Metabolism},
  author={Junzhe Zhao},
  journal={arXiv: Biomolecules},
  year={2017}
}
  • Junzhe Zhao
  • Published 20 December 2017
  • Biology, Computer Science
  • arXiv: Biomolecules
Hyperpolarized 13C-MRI allows real time observation of metabolism in vivo. Imaging sequences have been developed to follow the metabolism of [1-13C] pyruvate and extract reaction kinetics, which can show tumour treatment response. We applied the fitting model and algorithm for the imaging data of mice tumour models and determined error estimates for the parameters of interest. Data was least-squares fitted onto a two-site exchange model in MATLAB, followed by statistic computation to assess… 

References

SHOWING 1-10 OF 20 REFERENCES
Kinetic Modeling and Constrained Reconstruction of Hyperpolarized [1-13C]-Pyruvate Offers Improved Metabolic Imaging of Tumors.
TLDR
It is shown that metabolic MRI with hyperpolarized pyruvate is biased by tumor perfusion and a new pharmacokinetic model is presented that accounts for these effects and requires far fewer signal-depleting excitations, thus improving the reproducibility and accuracy of metabolic imaging measurements.
A comparison of quantitative methods for clinical imaging with hyperpolarized 13C‐pyruvate
TLDR
In this study, an in vitro system was used to compare several kinetic models, as well as simple model‐free methods, and appropriate quantitative methods were identified for future work in the analysis of human hyperpolarized 13C data.
Model Free Approach to Kinetic Analysis of Real-Time Hyperpolarized 13C Magnetic Resonance Spectroscopy Data
TLDR
A model-free formulism based on the ratio of total areas under the curve (AUC) of the injected and product metabolite, for example pyruvate and lactate is presented, which provides a robust and clinically relevant alternative to kinetic model-based rate measurements in the clinical translation of hyperpolarized 13C metabolic imaging in humans.
Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy
TLDR
In lymphoma-bearing mice injected intravenously with hyperpolarized [1-13C]pyruvate, it is shown that the lactate dehydrogenase–catalyzed flux of 13C label between the carboxyl groups of pyruvates and lactate in the tumor can be measured using 13C magnetic resonance spectroscopy and spectroscopic imaging, and that this flux is inhibited within 24 h of chemotherapy.
Kinetic modeling of hyperpolarized 13C pyruvate metabolism in tumors using a measured arterial input function
TLDR
This work presents a method for direct measurement of the AIF in the rat, using direct AIF rather than the commonly used box‐car AIF, and provides realistic estimates of the rate constant of conversion of pyruvate to lactate, kpl.
Effects of fasting on serial measurements of hyperpolarized [1‐13C]pyruvate metabolism in tumors
TLDR
Results indicate that the fasted state may be preferable for the measurement of 13C label exchange between pyruvate and lactate, as it reduces the variability and therefore should make it easier to detect the effects of therapy.
Optimizing Flip Angles for Metabolic Rate Estimation in Hyperpolarized Carbon-13 MRI
TLDR
This paper demonstrates through numerical simulation experiments that flip angles optimized based on the Fisher information lead to lower variance in metabolic rate estimates than previous flip angle sequences, and demonstrates appropriateness of the mathematical model used with in vivo experiments in a prostate cancer mouse model.
In vivo imaging of glucose uptake and metabolism in tumors
TLDR
The results of this study suggest that glucoCEST has potential as a useful and cost-effective method for characterizing disease and assessing response to therapy in the clinic.
Voxel‐by‐voxel correlations of perfusion, substrate, and metabolite signals in dynamic hyperpolarized 13C imaging
TLDR
Co‐administration of pyruvate with a dynamic nuclear polarization (DNP) perfusion agent is an effective approach for distinguishing between slow metabolism and poor perfusion and a practical strategy for lactate signal normalization to account for substrate delivery.
Single shot three‐dimensional pulse sequence for hyperpolarized 13C MRI
TLDR
A single‐shot three‐dimensional (3D) imaging sequence is described and its capability to generate 13C MR images in tumor‐bearing mice injected with hyperpolarized [1‐13C]pyruvate is demonstrated.
...
...