Kinetic Analysis of an Efficient DNA-Dependent TNA Polymerase

@inproceedings{Horhota2005KineticAO,
  title={Kinetic Analysis of an Efficient DNA-Dependent TNA
Polymerase},
  author={Allen Thomas Horhota and Keyong Zou and Justin K Ichida and Biao Yu and Larry W. McLaughlin and Jack W Szostak and John Charles Chaput},
  booktitle={Journal of the American Chemical Society},
  year={2005}
}
alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs). Therminator DNA polymerase, a variant of the 9 degrees N DNA polymerase, is an efficient DNA-directed threosyl nucleic acid (TNA) polymerase. Here we report a detailed kinetic comparison of Therminator-catalyzed TNA and DNA syntheses. We examined the rate of single-nucleotide incorporation for all… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 25 CITATIONS

Enzymatic Primer-Extension with Glycerol-Nucleoside Triphosphates on DNA Templates

VIEW 6 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

High fidelity TNA synthesis by Therminator polymerase

VIEW 5 EXCERPTS
CITES BACKGROUND, RESULTS & METHODS
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-6 OF 6 REFERENCES

J. Am. Chem. Soc

J K Ichida, K Zou, +3 authors J W Szostak
  • J. Am. Chem. Soc
  • 2005

Annu. ReV. Biochem. J. Mol. Biol

T A Kunkel, K Bebenek, +3 authors L A Besse
  • Annu. ReV. Biochem. J. Mol. Biol
  • 2000

Science Science

K.-U Schoning, P Scholz, +3 authors A Eschenmoser
  • Science Science
  • 1999

Cell J. W. Proc. Natl. Acad. Sci. U.S.A

M C Franklin, J Wang, T A Steitz, R W Roberts, Szostak
  • Cell J. W. Proc. Natl. Acad. Sci. U.S.A
  • 1997

For recent reviews

D S Wilson, J W Szostak, H Lin, V W Cornish
  • Curr. Opin. Struct. Biol. Annu. ReV. Biochem. (c) Breaker, R. R. Chem. ReV. Angew. Chem., Int. Ed
  • 1997

Nature Nature Nature Cell Nat. Struct. Biol

D L Ollis, P Brick, +21 authors M F Goodman
  • Nature Nature Nature Cell Nat. Struct. Biol
  • 1985

Similar Papers

Loading similar papers…