Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane.


The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.

DOI: 10.1016/j.bbrc.2009.11.068

Cite this paper

@article{Morton2010Kinesin1PA, title={Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane.}, author={April Morton and Anthony Lawrence Cunningham and Russell J Diefenbach}, journal={Biochemical and biophysical research communications}, year={2010}, volume={391 1}, pages={388-93} }