Kinematic changes during the cryopreservation of boar spermatozoa.

@article{Cremades2005KinematicCD,
  title={Kinematic changes during the cryopreservation of boar spermatozoa.},
  author={Teresa Cremades and Jordi Roca and Heriberto Rodr{\'i}guez-Mart{\'i}nez and Teresa Ab{\'a}igar and Juan Mar{\'i}a V{\'a}zquez and Emilio Arsenio Martinez},
  journal={Journal of andrology},
  year={2005},
  volume={26 5},
  pages={
          610-8
        }
}
The present study evaluates the effect that various steps of a conventional cycle of cryopreservation have on the patterns of movement exhibited by boar spermatozoa. Sperm-rich ejaculate fractions collected from 24 mature fertile boars (1 ejaculate per boar) were cryopreserved following a standard freeze-thaw procedure with 0.5-mL plastic straws. Overall sperm motility and the individual kinematic parameters of motile spermatozoa (assessed by the computer-aided sperm analysis system Sperm Class… 

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Controlled cooling during semen cryopreservation does not induce capacitation of spermatozoa from two portions of the boar ejaculate.

The data showed that kinetics, PMS and PMI of boar spermatozoa suspended in BTS, LEY or LEY plus glycerol were maintained during controlled cooling but were altered by thawing, showing more characteristics of cell injury than of sperm capacitation.

Boar semen can tolerate rapid cooling rates prior to freezing.

It is demonstrated that boar spermatozoa undergoing cryopreservation can withstand rapid cooling rates before freezing and exhibited slightly higher percentages of motile cells and intact plasma and acrosomal membranes in the samples that had been cooled slowly.
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