Kinematic changes during the cryopreservation of boar spermatozoa.

  title={Kinematic changes during the cryopreservation of boar spermatozoa.},
  author={Teresa Cremades and Jordi Roca and Heriberto Rodr{\'i}guez-Mart{\'i}nez and Teresa Ab{\'a}igar and Juan Mar{\'i}a V{\'a}zquez and Emilio Arsenio Martinez},
  journal={Journal of andrology},
  volume={26 5},
The present study evaluates the effect that various steps of a conventional cycle of cryopreservation have on the patterns of movement exhibited by boar spermatozoa. Sperm-rich ejaculate fractions collected from 24 mature fertile boars (1 ejaculate per boar) were cryopreserved following a standard freeze-thaw procedure with 0.5-mL plastic straws. Overall sperm motility and the individual kinematic parameters of motile spermatozoa (assessed by the computer-aided sperm analysis system Sperm Class… 

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Controlled cooling during semen cryopreservation does not induce capacitation of spermatozoa from two portions of the boar ejaculate.

The data showed that kinetics, PMS and PMI of boar spermatozoa suspended in BTS, LEY or LEY plus glycerol were maintained during controlled cooling but were altered by thawing, showing more characteristics of cell injury than of sperm capacitation.

Boar semen can tolerate rapid cooling rates prior to freezing.

It is demonstrated that boar spermatozoa undergoing cryopreservation can withstand rapid cooling rates before freezing and exhibited slightly higher percentages of motile cells and intact plasma and acrosomal membranes in the samples that had been cooled slowly.



Effects of centrifugation before freezing on boar sperm cryosurvival.

It is concluded that high g-force and short centrifugation time (3 minutes) do not affect sperm recovery and yield and that, moreover, they have a positive effect on the cryosurvival of boar sperm.

Comparison of the capacitation-like state of cooled boar spermatozoa with true capacitation.

Cooling spermatozoa to 5 degrees C results in a similar endpoint to that observed in capacitated cells in terms of reactive membranes and changes in intracellular ion concentrations, which may account for their comparable functionality.

Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene.

BHT had no effect on oocyte cleavage rates, but the development to blastocyst was improved for embryos derived from spermatozoa frozen in extender supplemented with 0.4 mM BHT.

Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis.

According to this multiparametric definition, induction of hyperactivity increased significantly (P < 0.0001) the fraction of hyperactive spermatozoa in semen samples and the threshold values of the following four parameters were well suited for differentiating between hyperactive and non-hyperactive boar spermatozosa.

Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials.

Results demonstrate that fertility information can be derived from the CASA analysis of boar semen provided it is combined with a period of incubation in capacitating conditions.

The causes of reduced fertility with cryopreserved semen.

Effect of seminal plasma on capacitation and hyperactivation in human spermatozoa.

The processes leading to hyperactivation and to the membrane changes associated with capacitation are not tightly interlinked and this finding is considered to be due to hyperactivated motility being associated with flagellar movement, while the CTC assay assesses changes in the Ca2+ levels of the sperm head plasma membrane.