Kindling fluorescent proteins for precise in vivo photolabeling

@article{Chudakov2003KindlingFP,
  title={Kindling fluorescent proteins for precise in vivo photolabeling},
  author={D. Chudakov and V. Belousov and A. Zaraisky and V. Novoselov and D. Staroverov and D. Zorov and S. Lukyanov and K. Lukyanov},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={191-194}
}
Photobleaching of green fluorescent protein (GFP) is a widely used approach for tracking the movement of subcellular structures and intracellular proteins. Although photobleaching is a powerful technique, it does not allow direct tracking of an object's movement and velocity within a living cell. Direct tracking becomes possible only with the introduction of a photoactivated fluorescent marker. A number of previous studies have reported optically induced changes in the emission spectra of… Expand
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