Kinase receptor activation (KIRA): a rapid and accurate alternative to end-point bioassays.

@article{Sadick1999KinaseRA,
  title={Kinase receptor activation (KIRA): a rapid and accurate alternative to end-point bioassays.},
  author={Michael D Sadick and Anthony Intintoli and Valerie E. Quarmby and Airlie J. McCoy and Eleanor Canova-Davis and Vincent Ling},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={1999},
  volume={19 6},
  pages={883-91}
}
We have developed a novel strategy for a rapid bioassay that is accurate, precise, sensitive, and high capacity. It is capable of quantifying ligand bioactivity by measuring ligand-induced receptor tyrosine kinase activation in terms of receptor phosphorylation. The assay, termed a 'kinase receptor activation' or KIRA, utilizes two separate microtiter plates, one for ligand stimulation of intact cells, and the other for receptor capture and phosphotyrosine ELISA. The assay makes use of either… CONTINUE READING