Killing of Coccidioides immitis by human peripheral blood mononuclear cells

  title={Killing of Coccidioides immitis by human peripheral blood mononuclear cells},
  author={Neil M. Ampel and G C Bejarano and John N. Galgiani},
  journal={Infection and Immunity},
  pages={4200 - 4204}
The ability of human peripheral blood mononuclear cells (MNL) obtained from healthy donors to kill the fungus Coccidioides immitis was examined in vitro with an assay that uses a single fungal particle per well. MNL killed 25.0% +/- 3.5% of a coccidioidal arthroconidial target, compared with the 4.7% +/- 2.9% killed by polymorphonuclear leukocytes obtained from the same donors (P = 0.012). Arthroconidial killing by MNL was not dependent on donor delayed dermal hypersensitivity to spherulin… 
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Alveolar macrophages from BALB/c mice readily phagocytized endospores and arthroconidia of Coccidioides immitis in vitro and treatment with rIFN-gamma activated the fungicidal capabilities of the alveolar Macrophages, as evidenced by the 50% reduction in the CFU.
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