Kidney aldose reductase gene transcription is osmotically regulated.

  title={Kidney aldose reductase gene transcription is osmotically regulated.},
  author={F L Smardo and Maurice B. Burg and Arlyn Garc{\'i}a-P{\'e}rez},
  journal={The American journal of physiology},
  volume={262 3 Pt 1},
Cells generally adapt to long-term hypertonic stress by accumulating organic osmolytes. PAP-HT25 renal medullary cells in hypertonic medium accumulate sorbitol through a reaction catalyzed by aldose reductase and betaine through osmotically regulated transport. Hypertonicity increases aldose reductase protein synthesis rate by elevating its mRNA abundance. To test whether the rise in aldose reductase mRNA is due to enhanced transcription, PAP-HT25 cells adapted to isotonic medium were switched… CONTINUE READING