Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ

@article{Wu2020KethoxalassistedSD,
  title={Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ},
  author={T. Wu and Ruitu Lyu and Qiancheng You and C. He},
  journal={Nature methods},
  year={2020},
  volume={17},
  pages={515 - 523}
}
  • T. Wu, Ruitu Lyu, +1 author C. He
  • Published 2020
  • Biology, Medicine
  • Nature methods
  • Transcription is a highly dynamic process that generates single-stranded DNA (ssDNA) in the genome as ‘transcription bubbles’. Here we describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach, based on the fast and specific reaction between N 3 -kethoxal and guanines in ssDNA. KAS-seq allows rapid (within 5 min), sensitive and genome-wide capture and mapping of ssDNA produced by transcriptionally active RNA polymerases or other processes in situ using as few as 1,000 cells… CONTINUE READING

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