Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area

@article{Ando2010KeratinocytesIC,
  title={Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area},
  author={Hideya Ando and Yoko Niki and Masaki Yoshida and Masaaki Ito and Kaoru Akiyama and Jin-Hwa Kim and Tae‐Jin Yoon and Jeung-Hoon Lee and Mary S. Matsui and Masamitsu Ichihashi},
  journal={Pigment Cell \& Melanoma Research},
  year={2010},
  volume={23}
}
There are many techniques for evaluating melanosome transfer to keratinocytes but the spectrophotometric quantification of melanosomes incorporated by keratinocyte phagocytosis has not been previously reported. Here we describe a new method that allows the spectrophotometric visualization of melanosome uptake by normal human keratinocytes in culture. Fontana‐Masson staining of keratinocytes incubated with isolated melanosomes showed the accumulation of incorporated melanosomes in the… 

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