Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin 5.

  title={Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin 5.},
  author={Zea Borok and Richard L. Lubman and Spencer I. Danto and X. L. Zhang and Stephanie M. Zabski and Landon Stuart King and D. M. Lee and Peter Agre and Edward D. Crandall},
  journal={American journal of respiratory cell and molecular biology},
  volume={18 4},
We investigated the role of keratinocyte growth factor (KGF) in regulation of alveolar epithelial cell (AEC) phenotype in vitro. Effects of KGF on cell morphology, expression of surfactant apoproteins A, B, and C (SP-A, -B, and -C), and expression of aquaporin 5 (AQP5), a water channel present in situ on the apical surface of alveolar type I (AT1) cells but not expressed in alveolar type II (AT2) cells, were evaluated in AECs grown in primary culture. Observations were made on AEC monolayers… 

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Modulation of pulmonary alveolar type II cell phenotype and communication by extracellular matrix and KGF.

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Expression and biological activity of ABCA1 in alveolar epithelial cells.

P pneumocytes with markers for the type I phenotype contained functional ABCA1 protein, released lipid to apoA-I protein, and were capable of producing particles resembling nascent high-density lipoprotein, indicating an important role for ABCA 1 in the maintenance of lung lipid homeostasis.

Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C

It is suggested that the culture may serve as a suitable model to study epithelial transport or cell biological processes in human alveolar cells to characterize a primary cell culture for in vitro model studying pulmonary drug delivery.

Phenotypic control of gap junctional communication by cultured alveolar epithelial cells.

Type II cells dynamically alter gap junctional communication, and distinct alveolar epithelial cell phenotypes express different connexins.



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The data indicate that the rate of rat type II AEC differentiation toward the type I cell phenotype is significantly modulated by soluble factor(s) present in rat serum.

Reversible transdifferentiation of alveolar epithelial cells.

It is concluded that commitment to the AT1 and AT2 cell lineages requires continuous regulatory input to maintain the differentiated states, and that transdifferentiation between AT2 and AT1 cells may be reversible.

Keratinocyte growth factor increases mRNAs for SP-A and SP-B in adult rat alveolar type II cells in culture.

It is concluded that keratinocyte growth factor can support differentiation of alveolar type II cells as well as act as a mitogen, thus suggesting an important role for KGF in maintenance of theAlveolar epithelium.

Keratinocyte growth factor and embryonic rat lung morphogenesis.

In conclusion, KGF markedly influences branching, and epithelial growth, differentiation, and patterning during lung morphogenesis.

Modulation of alveolar type II cell differentiated function in vitro.

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Keratinocyte growth factor is highly overexpressed in inflammatory bowel disease.

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KGF and EGF differentially regulate the phenotype of prostatic epithelial cells.

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