Keratinase of an anaerobic thermophilic bacterium Thermoanaerobacter sp. Strain 1004-09 isolated from a hot spring in the Baikal rift zone

Abstract

A thermophilic anaerobic bacterial strain 1004-09 belonging to the genus Thermoanaerobacter and capable of growth on protein substrates such as albumin, gelatin, casein, and α- and β-keratins was isolated from the Urinskii hot spring (Barguzin river valley, Republic of Buryatia, Russia). A 150-kDa serine proteinase was revealed in the strain supernatant; it exhibited optimal activity at 60°C and pH 9.3 and was capable of keratin hydrolysis. A number of characteristics for the strain 1004-09 keratinase were established including activation by SDS and NaCl and residual activity (15% to the activity of the intact protein) in the presence of 10% ethanol and acetone.

DOI: 10.1134/S0026261709010093

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@article{Kublanov2009KeratinaseOA, title={Keratinase of an anaerobic thermophilic bacterium Thermoanaerobacter sp. Strain 1004-09 isolated from a hot spring in the Baikal rift zone}, author={Ilya V. Kublanov and Kirill Tsiroulnikov and Elena N Kaliberda and Lev D. Rumsh and Thomas Haertl{\'e} and Elizaveta A. Bonch-Osmolovskaya}, journal={Microbiology}, year={2009}, volume={78}, pages={67-75} }