KdpFABC reconstituted in Escherichia coli lipid vesicles: substrate dependence of the transport rate.

@article{Damnjanovi2014KdpFABCRI,
  title={KdpFABC reconstituted in Escherichia coli lipid vesicles: substrate dependence of the transport rate.},
  author={Bojana Damnjanovi{\'c} and Hans-J{\"u}rgen Apell},
  journal={Biochemistry},
  year={2014},
  volume={53 35},
  pages={
          5674-82
        }
}
KdpFABC complexes were reconstituted in Escherichia coli lipid vesicles, and ion pumping was activated by addition of ATP to the external medium which corresponds to the cytoplasm under physiological conditions. ATP-driven potassium extrusion was studied in the presence of various substrates potentially influencing transport rate. The pump current was detected as a decrease of the membrane potential by the voltage-sensitive dye DiSC3(5). The results indicate that high cytoplasmic K… Expand
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