Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG) n repeat in eight species of true bugs (Hemiptera, Heteroptera)

  title={Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG) n repeat in eight species of true bugs (Hemiptera, Heteroptera)},
  author={Snejana M Grozeva and Valentina G. Kuznetsova and Boris Anokhin},
  journal={Comparative Cytogenetics},
  pages={355 - 374}
Abstract Eight species belonging to five true bug families were analyzed using DAPI/CMA3-staining and fluorescence in situ hybridization (FISH) with telomeric (TTAGG)n and 18S rDNA probes. Standard chromosomal complements are reported for the first time for Deraeocoris rutilus (Herrich-Schäffer, 1838) (2n=30+2m+XY) and Deraeocoris ruber(Linnaeus, 1758) (2n=30+2m+XY) from the family Miridae. Using FISH, the location of a 18S rDNA cluster was detected in these species and in five more species… 

Figures and Tables from this paper

Detection of telomeric sequences and ribosomal RNA genes in holokinetic chromosomes of five jumping plant-lice species: First data on the superfamily Psylloidea (Hemiptera: Sternorrhyncha)

It is found that the chromosomes of all studied species contain the insect-type telomere motif, (TTAGG)n, and is speculated, based on the obtained FISH markers, on the mechanisms of karyotype evolution of psylloid species differing in chromosome numbers and sex chromosome systems.

Chromosomal distribution of interstitial telomeric sequences as signs of evolution through chromosome fusion in six species of the giant water bugs (Hemiptera, Belostoma)

The ITS distribution between these closely related species supports the hypothesis that several telomere–telomere fusions of the chromosomes from an ancestral diploid chromosome number played a major role in the karyotype evolution of Belostoma.

New evidence for the presence of the telomere motif (TTAGG) n in the family Reduviidae and its absence in the families Nabidae and Miridae (Hemiptera, Cimicomorpha).

Increasing evidence reinforces the hypothesis of the loss of the canonical "insect" telomere motif (TTAGG)n by at least four cimicomorphan families, Nabidae, Miridae, Tingidae, and Cimicidae, for which data are currently available.

Comparative FISH mapping of ribosomal DNA clusters and TTAGG telomeric sequences to holokinetic chromosomes of eight species of the insect order Psocoptera

This is the first study of rDNA clusters in the order Psocoptera using FISH and shows that all the examined species share a similar location of 18S rDNA on a medium-sized pair of autosomes.

Cytogenetic Characterization of Eight Odonata Species Originating from the Curonian Spit (the Baltic Sea, Russia) Using C-Banding and FISH with 18S rDNA and Telomeric (TTAGG)n Probes

More work needs to be done to answer questions about the organization of telomeres in this very ancient and thus phylogenetically important insect order, and the possibility that in Odonata the canonical “insect” telomeric repeat is in fact present but in very low copy number and is consequently difficult to detect by in situ hybridization is raised.

Karyotype stability in the family Issidae (Hemiptera, Auchenorrhyncha) revealed by chromosome techniques and FISH with telomeric (TTAGG)n and 18S rDNA probes

Several chromosomal traits in 11 species from 8 genera of the planthopper family Issidae, the tribes Issini, Parahiraciini and Hemisphaeriini are reported, suggesting that chromosomal rearrangements involving the rDNA segments occurred in the evolution of the family.

Ribosomal DNA clusters and telomeric (TTAGG)n repeats in blue butterflies (Lepidoptera, Lycaenidae) with low and high chromosome numbers

First cytogenetic study of ribosomal DNA clusters and telomeric repeats in seven blue butterflies of the genus Polyommatus Latreille with low and high chromosome numbers is presented using fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomere probes.

Karyotypes diversity in some Iranian Pamphagidae grasshoppers (Orthoptera, Acridoidea, Pamphagidae): new insights on the evolution of the neo-XY sex chromosomes

It is hypothesized that the origin and early phase of evolution of the neo-Xneo-Y♂ sex chromosome in the subfamily Pamphaginae, are linked to the Iranian highlands.

First evidence for (TTAGG)n telomeric sequence and sex chromosome post-reduction in Coleorrhyncha (Insecta, Hemiptera)

It is shown that Peloridium pomponorum displays 2n = 31 (30A + X) in males, the classical insect (TTAGG)n telomere organization and sex chromosome post-reduction during spermatocyte meiosis.

The conservation of number and location of 18S sites indicates the relative stability of rDNA in species of Pentatomomorpha (Heteroptera).

The diversity in the number of species analyzed here showed a tendency of 18S rDNA to remain among the autosomes, except for Leptoglossus gonagra and Euryophthalmus rufipennis, which showed signals in the m- and sex chromosomes, respectively.



Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)

The results led to propose that the location of rDNA genes could be associated with variants of the sex chromosome systems in relation with a kind of thesex chromosome systems within this family.

Bed bug cytogenetics: karyotype, sex chromosome system, FISH mapping of 18S rDNA, and male meiosis in Cimex lectularius Linnaeus, 1758 (Heteroptera: Cimicidae)

A cytogenetic study of the common bed bug Cimex lectularius Linnaeus, 1758 is performed using both classical (Schiff-Giemsa and AgNO 3 -staining) and mo- lecular (base-specifi c DAPI/CMA 3 fl uorochromes and FISH with an 18S rDNA probe).

Chromosomal location of heterochromatin and 45S rDNA sites in four South American triatomines (Heteroptera: Reduviidae)

The karyotype of four species of the genus Triatoma Laporte, 1832 is determined using chromosome measurement, chromosome banding and FISH with a 45S rDNA probe to suggest a closer relationship between T. brasiliensis, T. matogrossensis and T. rubrovaria.

TTAGG Telomeric Repeats in Chromosomes of Some Insects and Other Arthropods

The results, which confirm and extend earlier observations, suggest that (TTAGG)n was a phylogenetically ancestral telomere motif in the insect lineage but was lost independently in different groups, being replaced probably by other telomeres motifs.

Phylogenetic distribution of TTAGG telomeric repeats in insects.

The repeated losses of TTAGG in different branches of the insect phylogenetic tree and, in particular, in the most successful lineage of insect evolution, the Endopterygota, suggest a backup mechanism in the genome of insects that enabled them frequent evolutionary changes in telomere composition.

First evidence of sex chromosome pre-reduction in male meiosis in the Miridae bugs (Heteroptera).

The karyotype and male meiosis of Macrolophus costalis Fieber (Insecta, Heteroptera, Miridae) were studied using C- banding, AgNOR-banding and DNA sequence specific fluorochrome staining to find the first evidence of sex chromosome pre-reduction in the family Miridae.

Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization.

The data suggest that in triatomines, in general, the number and location of NORs are species-specific and these regions may be considered important chromosome markers for comparative studies to improve the understanding of evolutionary mechanisms in these hematophagous insects.

Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

The C-band pattern in individuals of Holhymenia rubiginosa from different populations collected in different years is analyzed and it is suggested that heterochromatin should be selectively neutral in H. rubIGinosa.

Male meiotic behaviour and nucleolus organizer regions in Camptischium clavipes (Fabr.) (Coreidae, Heteroptera) analyzed by fluorescent banding and in situ hybridization

The NOR is used as a cytological marker and the observations give further support to the hypothesis on the alternate kinetic activity of telomeric regions of autosomes at meiosis I and II in Heteroptera.

Sex Chromosome Evolution in Cotton Stainers of the Genus Dysdercus (Heteroptera: Pyrrhocoridae)

The hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system is supported, as a consequence, the homologue of this NOR-autosome became the Neo-Y chromosome.