Karyometry and quantitative immunohistochemical analysis of the urothelium in tissue sections: a feasibility study based on chromatin remodeler DAXX immunostaining.

@article{Zizzi2013KaryometryAQ,
  title={Karyometry and quantitative immunohistochemical analysis of the urothelium in tissue sections: a feasibility study based on chromatin remodeler DAXX immunostaining.},
  author={Antonio Zizzi and Maria Alessandra Montironi and Roberta Mazzucchelli and Marina Scarpelli and Antonio Lopez-Beltran and Liang Cheng and Roberto Di Primio and Nicola Paone and Paolo Castellini and Rodolfo Montironi},
  journal={Journal of biological regulators and homeostatic agents},
  year={2013},
  volume={27 3},
  pages={913-7}
}
The aim of this study was to investigate the feasibility of applying a software traditionally used in the field of engineering to pathology, in particular to tissue sections from normal urothelium (NU) immuno-stained for the chromatin remodeler DAXX (death domain-associated protein). The study included 5 cases of NU. Images were recorded with a Nikon digital camera. The nuclear area and the intensity of nuclear staining were analyzed with a software package developed in LabVIEW environment. The… CONTINUE READING