Kanamycin resistance as a selectable marker for plastid transformation in tobacco

@article{Carrer1993KanamycinRA,
  title={Kanamycin resistance as a selectable marker for plastid transformation in tobacco},
  author={Helaine Carrer and Tish Noel Hockenberry and Z{\'o}ra Sv{\'a}b and Pal Maliga},
  journal={Molecular and General Genetics MGG},
  year={1993},
  volume={241},
  pages={49-56}
}
We report on a novel chimeric gene that confers kanamycin resistance on tobacco plastids. The kan gene from the bacterial transposon Tn5, encoding neomycin phosphotransferase (NPTII), was placed under control of plastid expression signals and cloned between rbcL and ORF512 plastid gene sequences to target the insertion of the chimeric gene into the plastid genome. Transforming plasmid pTNH32 DNA was introduced into tobacco leaves by the biolistic procedure, and plastid transformants were… CONTINUE READING
BETA

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 79 CITATIONS, ESTIMATED 77% COVERAGE

Efficient plastid transformation in tobacco using the aphA-6 gene and kanamycin selection

  • Molecular Genetics and Genomics
  • 2002
VIEW 6 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Site-specific recombinases to manipulate the plastid genome

VIEW 4 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

Cucumber: a model angiosperm for mitochondrial transformation?

VIEW 3 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

1994
2018

CITATION STATISTICS

  • 7 Highly Influenced Citations

References

Publications referenced by this paper.
SHOWING 1-10 OF 29 REFERENCES

High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1993
VIEW 5 EXCERPTS
HIGHLY INFLUENTIAL