KRAS mutation: comparison of testing methods and tissue sampling techniques in colon cancer.

@article{Franklin2010KRASMC,
  title={KRAS mutation: comparison of testing methods and tissue sampling techniques in colon cancer.},
  author={Wilbur A. Franklin and J. S. Haney and Michio Sugita and Lynne T. Bemis and Antonio J. Jimeno and Wells A. Messersmith},
  journal={The Journal of molecular diagnostics : JMD},
  year={2010},
  volume={12 1},
  pages={43-50}
}
Treatment of colon carcinoma with the anti-epidermal growth factor receptor antibody Cetuximab is reported to be ineffective in KRAS-mutant tumors. Mutation testing techniques have therefore become an urgent concern. We have compared three methods for detecting KRAS mutations in 59 cases of colon carcinoma: 1) high resolution melting, 2) the amplification refractory mutation system using a bifunctional self-probing primer (ARMS/Scorpion, ARMS/S), and 3) direct sequencing. We also evaluated the… CONTINUE READING