KGF increases SP-A and SP-D mRNA levels and secretion in cultured rat alveolar type II cells.

@article{Xu1998KGFIS,
  title={KGF increases SP-A and SP-D mRNA levels and secretion in cultured rat alveolar type II cells.},
  author={Xiaobin Xu and Kathleen McCormick-Shannon and Dennis R. Voelker and Robert J. Mason},
  journal={American journal of respiratory cell and molecular biology},
  year={1998},
  volume={18 2},
  pages={168-78}
}
Studies of secretion of surfactant proteins by alveolar type II cells have been limited because the expression of the genes for these proteins decreases rapidly in primary culture. We developed a culture system to investigate the regulation of lipid and protein secretion by alveolar type II cells and the genes involved in these processes. Rat type II cells were plated on membrane inserts coated with rat-tail collagen in medium containing 10% fetal bovine serum (FBS) for 1 d before being changed… CONTINUE READING

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