KF polymerase-based fluorescence aptasensor for the label-free adenosine detection.

@article{Liao2012KFPF,
  title={KF polymerase-based fluorescence aptasensor for the label-free adenosine detection.},
  author={Dongli Liao and Huping Jiao and Bin Wang and Quan Lin and Cong Yu},
  journal={The Analyst},
  year={2012},
  volume={137 4},
  pages={978-82}
}
We have developed a simple, inexpensive, and label-free method for the selective detection of adenosine. Klenow fragment polymerase (KF polymerase) is a commonly-used 5' to 3' DNA polymerase, it also has 3' to 5' exonuclease activity that can digest single-stranded DNA. An adenosine binding DNA aptamer was employed, the aptamer was split into two pieces of single-stranded DNA (aptamer-A1 + aptamer-A2). Without the addition of adenosine, aptamer-A1 and aptamer-A2 existed as single-stranded DNA… CONTINUE READING

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