K-ras codon 12 mutations in biliary tract tumors detected by polymerase chain reaction denaturing gradient gel electrophoresis.

Abstract

BACKGROUND Although the prevalence of K-ras codon 12 mutations in biliary tract (BT) tumors has been addressed in previous studies, the results have shown large discrepancies in mutation frequency. METHODS K-ras codon 12 mutations were investigated by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE), a sensitive method for… (More)

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