K-252a, a novel microbial product, inhibits smooth muscle myosin light chain kinase.

@article{Nakanishi1988K252aAN,
  title={K-252a, a novel microbial product, inhibits smooth muscle myosin light chain kinase.},
  author={Satoshi Nakanishi and Kazunori D. Yamada and Hiroshi Kase and Satsuki Nakamura and Yoshiaki Nonomura},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 13},
  pages={6215-9}
}
Effects of K-252a, (8R*, 9S*, 11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8, 11-epoxy-1H,8H,11H-2,7b,11a-triazadibenzo[a,g]cycloocta [cde]trinden-1-one, purified from the culture broth of Nocardiopsis sp., on the activity of myosin light chain kinase were investigated. 1) K-252a (1 x 10(-5) M) affected three characteristic properties of chicken gizzard myosin-B, natural actomyosin, to a similar degree: the Ca2+-dependent activity of ATPase, superprecipitation, and the… CONTINUE READING