Joint determination by Brownian dynamics and fluorescence quenching of the in-depth location profile of biomolecules in membranes.

@article{Fernandes2002JointDB,
  title={Joint determination by Brownian dynamics and fluorescence quenching of the in-depth location profile of biomolecules in membranes.},
  author={Miguel X. Fernandes and Jos{\'e} Garc{\'i}a de la Torre and Miguel A. R. B. Castanho},
  journal={Analytical biochemistry},
  year={2002},
  volume={307 1},
  pages={1-12}
}
The in-depth molar distribution function of fluorophores is revealed by a new methodology for fluorescence quenching data analysis in membranes. Brownian dynamics simulation was used to study the in-depth location profile of quenchers. A Lorentzian profile was reached. Since the Stern-Volmer equation is valid at every depth in the membrane for low quencher concentrations, the molar distribution of the fluorophore (also regarded as a Lorentzian) can be achieved. The average location and the… CONTINUE READING