Japanese Eel Follicle-Stimulating Hormone (Fsh) and Luteinizing Hormone (Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology1

@inproceedings{Kazeto2008JapaneseEF,
  title={Japanese Eel Follicle-Stimulating Hormone (Fsh) and Luteinizing Hormone (Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology1},
  author={Y. Kazeto and M. Kohara and T. Miura and C. Miura and S. Yamaguchi and J. Trant and S. Adachi and K. Yamauchi},
  booktitle={Biology of reproduction},
  year={2008}
}
Abstract Two gonadotropins (Gths), follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), control gonadal steroidogenesis and gametogenesis in vertebrates, including teleost fish. Here, we report on the production of biologically active recombinant Fsh (rec-Fsh) and Lh (rec-Lh) in Japanese eel using Drosophila S2 cells. The three subunits composing Gths, i.e., glycoprotein hormone, alpha polypeptide (Cga), follicle-stimulating hormone, beta polypeptide (Fshb), and luteinizing hormone… Expand
Follicle-Stimulating Hormone and Luteinizing Hormone Mediate the Androgenic Pathway in Leydig Cells of an Evolutionary Advanced Teleost1
TLDR
Fsh-mediated androgen production through the activation of cognate receptors in Leydig cells is a conserved pathway in Senegalese sole, suggesting that activation of steroidogenesis through the cAMP/PKA pathway is the major route for both Lh- and Fsh-stimulated androgen secretion. Expand
Functional analysis of recombinant single-chain Japanese eel Fsh and Lh produced in FreeStyle 293-F cell lines: binding specificities to their receptors and differential efficacy on testicular steroidogenesis.
TLDR
Functional analyses of reFsh and reLh revealed that both eel Fsh and Lh acted as steroidogenic hormones through their receptors in testicular somatic cells; however, Lh was more potent on androgen production, implying differential functions on spermatogenesis. Expand
Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane.
TLDR
It is demonstrated that 17β-estradiol (E2), but not testosterone, was a potent endocrine hormone that differentially regulated the expression of fshr and lhcgr and sheds important light on the functions of the two GTH and their receptors in fish as well as the conservation and diverse aspects of GTHR regulation across vertebrates. Expand
Characterization, Localization, and Stage-Dependent Gene Expression of Gonadotropin Receptors in Chub Mackerel (Scomber japonicus) Ovarian Follicles1
TLDR
Findings suggest that switching of the expression of F SHR to LHR controls the effects of FSH and/or LH on vitellogenesis and final oocyte maturation via steroid production in granulosa and thecal cells. Expand
Development and validation of enzyme-linked immunosorbent assays specific for follicle-stimulating hormone and luteinizing hormone in Japanese eel
TLDR
Recombinant Gths and homologous ELISAs established for Japanese eel may be useful tools for studying reproductive eel biology and specific enzyme-linked immunosorbent assays forJapanese eel Fsh and Lh were developed for the first time. Expand
Molecular Characterization and Gene Expression of Japanese Eel (Anguilla japonica) Gonadotropin Receptors
TLDR
Data on gene expression and ligand-activation of Gthrs suggest that Fsh and Lh act differentially in the regulation of reproductive function in Japanese eel. Expand
Expressional regulation of gonadotropin receptor genes and androgen receptor genes in the eel testis.
TLDR
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TLDR
Recombinant bovine FSH (bFSHβα) was produced from bacteria and yeast heterologous expression systems, and the ovarian weight gain biological activity assay was performed, suggesting the recombinant protein retains biological activity. Expand
Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka
TLDR
It is demonstrated that Lh/Lhcgrbb is critically involved in the induction of oocyte maturation and ovulation and was previously demonstrated to be crucial for ovulation in the fish. Expand
Expression of gonadotropin subunit and gonadotropin receptor genes in wild female New Zealand shortfinned eel (Anguilla australis) during yellow and silver stages.
TLDR
The data suggest that Fsh release may be very limited, or absent, prior to onset of puberty in shortfinned eels and that Lh is not functionally important in this fish during the EV stage. Expand
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TLDR
The objective of the present study was to express glycosylated, properly folded, and biologically active tilapia FSH (tFSH) using the Pichia pastoris expression system to develop a specific ELISA and to enable the study of FSH response to GnRH. Expand
Zebrafish Gonadotropins and Their Receptors: II. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Subunits—Their Spatial-Temporal Expression Patterns and Receptor Specificity1
TLDR
The present study systematically investigated gonadotropins in the zebrafish in terms of their structure, spatial-temporal expression patterns, and receptor specificity, providing a solid foundation for further studies on the physiological relevance of FSH and LH in theZebrafish, one of the top biological models in vertebrates. Expand
Molecular biology and biochemistry of human recombinant follicle stimulating hormone (Puregon).
TLDR
It is shown that FSH-gene transfected CHO-cells are capable of stable serum-free production of recombinant FSH, and a process has been developed which assures the consistent and reproducible production of highly-purified recombinantFSH. Expand
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TLDR
Results indicate that LH is the determining factor regulating the production of the maturation-inducing steroid, 17,20β-P, and the induction of GVBD in the salmonid ovary, which further supports the hypothesis that FSH and LH have distinct functions in the teleost ovary. Expand
Follicle-Stimulating Hormone Induces Spermatogenesis Mediated by Androgen Production in Japanese Eel, Anguilla japonica1
TLDR
The results clearly show that the main function of FSH in eel is to induce spermatogenesis via stimulating androgen production. Expand
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TLDR
These recombinant GtHs activated their receptors in vitro, enhanced estrogen secretion, up-regulated several steroidogenic enzyme genes in channel catfish ovarian follicles, and increased androgen secretion from African catfish testis. Expand
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TLDR
The production of biologically-active recombinant FSH and LH of an endangered salmon species, Manchurian trout, by baculovirus in silkworm larvae is reported, suggesting that these recombinant hormones have distinct biological properties, such as a specific responsiveness for the cognate receptor, steroidogenic, and vitellogenic activities for ovarian follicles in salmonids. Expand
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TLDR
Results suggest that eel FSH is a heterodimeric molecule which consists of distinct glycoprotein subunits, GPalpha and FSHbeta, which is presumably stimulating the gametogenesis through the sex steroid secretion during the early stages of gonadal development. Expand
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TLDR
Recombinant LH (rLH) is likely to be recommended as a supplement to rFSH for ovulation induction in hypogonadotropic women, and Recombinant HCG appears to be as efficacious as urinary HCG with the benefit of improved local tolerance. Expand
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TLDR
Intact FSH and LH showed gonadotropic activity by stimulating release of 11-ketotestosterone from turbot (Scophthalmus maximus L.) testicular tissue in vitro, providing the first purification of two distinct GtHs from an evolutionary advanced pleuronectiform teleost. Expand
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