author={Yvonne Nemcova and Marek Eli{\'a}{\vs} and Pavel {\vS}kaloud and Ladislav Hoda{\vc} and Jiř{\'i} Neustupa},
  journal={Journal of Phycology},
The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope… 

Lunachloris lukesovae gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid green alga isolated from soil in South Bohemia, Czech Republic

The phylogenetic position, morphology and ultrastructure of the strain CCALA 307 previously identified as Coccomyxa cf.

A new species Jenufa aeroterrestrica (Chlorophyceae incertae sedis, Viridiplantae), described from Europe

  • Biology
  • 2015
This paper describes and characterize a new species of the genus Jenufa, J. aeroterrestrica, which was identified in five samples of corticolous microalgal biofilms collected in Europe, and indicates that the newly described species frequently occurs in temperate and sub-Mediterranean European subaerial bio Films, such as those occurring on tree bark or surfaces of stone buildings.

Obliquicauda gen. nov. (Trebouxiophyceae, Chlorophyta), including O. inflata sp. nov. and O. apiculata sp. nov.: foliicolous algae from Ficus leaves

Morphological, ultrastructural, and molecular phylogenetic investigations of two unidentified foliicolous algal strains in a tropical rainforest of China indicated that they differ from each other but cluster together and represent a new lineage of Trebouxiophyceae in the Choricystis/Botryococcus clade.

Chloropyrula uraliensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a new green coccoid alga with a unique ultrastructure, isolated from soil in South Urals

An interesting coccoid green alga isolated from a soil sample from a forest‐steppe in South Urals (Bashkortostan, Russia) is described that appears to represent a new phylogenetic lineage related to the genus Leptosira within the class Trebouxiophyceae.

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats.

This study provides a detailed characterization of two strains of microalgae isolated from tree bark in the Mediterranean, described as representatives of a novel species in a new genus, Leptochlorella corticola gen. et sp.

Molecular diversity of green corticolous microalgae from two sub-Mediterranean European localities

The samples taken from oak trees were more diverse in their OTU composition than those taken from pine trees, but the average phylogenetic distances of OTUs in samples did not differ between the host tree taxa, indicating that habitat filtering is important for the distribution of individual microalgal phylogenetic taxa.

Molecular Evidence for the Wide Distribution of Two Lineages of Terrestrial Green Algae (Chlorophyta) over Tropics to Temperate Zone

Phylogenetic analyses of 18S rDNA sequences from environmental clones and culture strains revealed a widespread distribution of two subaerial green algal lineages, Jenufa and Xylochloris, recently

Chloroplast phylogenomic analysis of chlorophyte green algae identifies a novel lineage sister to the Sphaeropleales (Chlorophyceae)

A chloroplast phylogenomic study advances knowledge regarding the circumscription and internal structure of the Chlamydomonadales, suggesting that a previously unrecognized lineage is sister to the Sphaeropleales.

Morphology and Phylogenetic Position of the Freshwater Green Microalgae Chlorochytrium (Chlorophyceae) and Scotinosphaera (Scotinosphaerales, ord. nov., Ulvophyceae)

The close relationship of the Scotinosphaerales with other early diverging ulvophycean orders enforces the notion that nonmotile unicellular freshwater organisms have played an important role in the early diversification of the Ulvophyceae.

Probing the Monophyly of the Sphaeropleales (Chlorophyceae) Using Data From Five Genes

A molecular phylogenetic study of 16 accessions representing all known families and diverse affiliated lineages within the order Sphaeropleales resolved Volvocales, algae possessing predominantly biflagellate motile cells with clockwise flagellar orientation, as the monophyletic sister lineage to Sphaerropleales, and an alliance of Chaetopeltidales, Chaetophorales,



Hylodesmus singaporensis gen. et sp. nov., a new autosporic subaerial green alga (Scenedesmaceae, Chlorophyta) from Singapore.

The results establish that the strain identified is a novel, previously undocumented, evolutionary lineage of scenedesmacean algae necessitating its description as a new species in a new genus.

Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis , with the description of a new genus and species

A novel unicellular Chlorella-like green alga was isolated from the rubber tree (Hevea brasiliensis) and based on morphological features and phylogenetic position, a new genus Heveochlorella gen. nov. is proposed.

Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella‐like subaerial alga from Southeast Asia

A new subaerial Chlorella‐like strain CAUP H7901 is studied and morphological, ultrastructural, and molecular phylogenetic investigations indicated that it represents a new lineage of the trebouxiophycean Watanabea clade, dissimilar from other members of this group.

Morphology and phylogenetic position of Spongiochrysis hawaiiensis gen. et sp. nov., the first known terrestrial member of the order Cladophorales (Ulvophyceae, Chlorophyta).

Phylogenetic analyses of the 18S rRNA gene showed that Spongiochrysis hawaiiensis is a member of the freshwater clade of the Cladophorales/Siphonocladales lineage in the green algal class Ulvophyceae and can be considered as the first known successful step of a members of the order Cladphorales into terrestrial habitats.

Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga

Two novel tropical strains found to be an independent lineage without an obvious sister group are described as representatives of a new trebouxiophycean genus and species, Xylochloris irregularis gen. et sp.


It is proposed that only four species should be kept in the genus Chlorella (Chlorophyta, Trebouxiophyceae), and these taxa can easily be recognized by the production of secondary carotenoids under nitrogen‐deficient conditions.

Systematics of the green algae: Conflict of classic and modern approaches

Phylogenetic analyses of nuclearencoded SSU and ITS rDNA sequences have basically confirmed the classification based on ultrastructural characters of green algae according to morphological species concept.

Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for "budding" subaerial green algae Marvania aerophytica NEUSTUPA et ŠEJNOHOVÁ and Stichococcus ampulliformis HANDA.

It is demonstrated that M. aerophytica is unrelated to other species of the genus Marvania, but together with S. ampulliformis forms a lineage within a broader clade comprising also Species of the order Prasiolales, Desmococcus spp.

Phylogenetic position of someChlorella species within the chlorococcales based upon complete small-subunit ribosomal RNA sequences

Complete small-subunit rRNA (16S-like rRNA) coding region sequences were determined for eight species of the Chlorococcales (Chlorophyceae) anddistance matrix methods were used to infer a phylogenetic tree that describes evolutionary relationships between several plant and green algal groups.

Phylogenetic position of Ooplanctella planoconvexa gen. et comb. nova and Echinocoleum elegans (Oocystaceae, Trebouxiophyceae, Chlorophyta).

Molecular analysis of the 18S rRNA gene of C. planoconvexa CAUP H 5502 and E. elegans SAG 37.93 revealed that they both belong to Oocystaceae, however, the two strains were not a part of a monophyletic cluster.