J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential.

Abstract

The spectral properties of a novel membrane potential sensitive probe (JC-1) were characterized in aqueous buffers and in isolated cardiac mitochondria. JC-1 is a carbocyanine with a delocalized positive charge. It formed under favorable conditions a concentration-dependent fluorescent nematic phase consisting of J-aggregates. When excited at 490 nm, the… (More)

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Cite this paper

@article{Reers1991JaggregateFO, title={J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential.}, author={Martin Reers and Trevor Smith and L. B. Chen}, journal={Biochemistry}, year={1991}, volume={30 18}, pages={4480-6} }