Iterative selection from a Salmonella typhimurium cosmid library can lead to the isolation of an atypically small plasmid.


We have constructed a DNA library from a virulent Salmonella typhimurium strain, in an avirulent strain. The process of selecting the components of interest from the library involved iterative growth in liquid culture. This resulted, after four cycles, in the culture becoming homogeneous for a single plasmid, which was much smaller than the average size for… (More)


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