Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

@article{Reetz2007IterativeSM,
  title={Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes},
  author={Manfred T. Reetz and Jos{\'e} Daniel Carballeira},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={891-903}
}
Iterative saturation mutagenesis (ISM) is a new and efficient method for the directed evolution of functional enzymes. It reduces the necessary molecular biological work and the screening effort drastically. It is based on a Cartesian view of the protein structure, performing iterative cycles of saturation mutagenesis at rationally chosen sites in an enzyme, a given site being composed of one, two or three amino acid positions. The basis for choosing these sites depends on the nature of the… Expand
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