Corpus ID: 83581270

Israeli Acute Paralysis Virus (IAPV) 검출을 위한 Semi-nested PCR 법의 개발

@article{2010IsraeliAP,
  title={Israeli Acute Paralysis Virus (IAPV) 검출을 위한 Semi-nested PCR 법의 개발},
  author={노지나 and Nguyen Van Phu and 유미선 and 윤병수},
  journal={Journal of Apiculture},
  year={2010},
  volume={25},
  pages={97-103}
}
A new semi-nested PCR method was developed to detect Israeli acute paralysis virus (IAPV) which is the causative virus of colony collapse disorder (CCD) in honeybee. To amplify IAPV structural protein gene, the sequences of IAPV including accession No. ER219380, EU436455, EU436456, EU224279, EU224280, EU436423, EU218534 in GenBank were analysed. Two pairs of IAPV specific primer sets named IAPV_F1/2, IAPV_R1 outer and IAPV_F1/2, IAPV_R2 inner were designed and evaluated. As results, the unique… Expand
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