Isozymes of human erythrocyte pyrimidine 5'-nucleotidase--chromatographic separation and their properties.

@article{Hirono1985IsozymesOH,
  title={Isozymes of human erythrocyte pyrimidine 5'-nucleotidase--chromatographic separation and their properties.},
  author={Akira Hirono and Hisaichi Fujii and Shiro Miwa},
  journal={The Japanese journal of experimental medicine},
  year={1985},
  volume={55 1},
  pages={37-44}
}
The biochemical properties of two isozymes of human erythrocyte pyrimidine 5'-nucleotidase (P5N) separated by ion-exchange chromatography were studied. These two isozymes, P5N-I and P5N-IIB, can be distinguished from each other by substrate specificity, pH dependent activity and thermostability. P5N-I is thermolabile and dephosphorylates various pyrimidine 5'-monophosphates optimally around pH 7. UMP and CMP were found to be most effective substrates. P5N-IIB is thermostable and characterized… CONTINUE READING