Isozyme shift in cultured fetal human hepatocytes: a study of pyruvate kinase and phosphofructokinase.

@article{GuguenGuillouzo1980IsozymeSI,
  title={Isozyme shift in cultured fetal human hepatocytes: a study of pyruvate kinase and phosphofructokinase.},
  author={Christiane Guguen-Guillouzo and Joělle Marie and Dominique Cottreau and Nicole Pasdeloup and Axel Kahn},
  journal={Biochemical and biophysical research communications},
  year={1980},
  volume={93 2},
  pages={
          528-34
        }
}
Abstract Hepatocytes from a 4-month old fetus were cultured for 15 days. We found that fetal hepatocytes contained some R1 (precursor) form of L-type pyruvate kinase. Culture was associated with a considerable increase of the M2-type pyruvate kinase activity, but some L-type enzyme could be detected even after 10 days. Isozyme shift of phosphofructokinase seemed to be a progressive rather low phenomenon. Fetal hepatocytes showed an increase of the F-type form and a disappearance of the M-type… Expand
13 Citations
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The results demonstrate that EH and AHH activities in HFH cultures are inducible by classical in vivo inducers, and it is plausible that such induction takes place also in intrauterine life. Expand
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Abstract Human fetal liver RNA translated in a rabbit reticulocyte cell-free system directed synthesis of two polypeptides which could be identified by immunological competition as L and L′ pyruvateExpand
Regulation of Pyruvate Kinase Gene Expression by Hormones and Developmental Factors
These are exciting times in the development of our knowledge of the regulation of an important glycolytic enzyme—pyruvate kinase. A series of excellent laboratories are working in this area. ThisExpand
Modulation of Specific Functions in Adult and Fetal Hepatocytes Maintained in a Co-Cultured System
When co-cultured with another epithelial cell type derived from rat liver, human and rat hepatocytes can survive for several weeks in a serum-free medium. Adult parenchymal cells retained specificExpand
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