Isozyme analysis of human normal polymorphonuclear leukocyte phosphofructokinase.

  title={Isozyme analysis of human normal polymorphonuclear leukocyte phosphofructokinase.},
  author={P Durante and Xiomara Raleigh and M E G{\'o}mez and Gilberto Campos and Elena Ryder},
  journal={Biochemical and biophysical research communications},
  volume={216 3},
Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies, column chromatography on QAE-Sephadex and SDS-polyacrylamide gel electrophoresis. Two different isozymes, M-type and L-type, were found. The M(r) values of the M and L subunits were 79,500 +/- 1,914 and 74,250 +/- 1,258, respectively. The two isozymes presented different kinetic and regulatory properties. The results suggest that PFK from human… Expand
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