Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality.

Abstract

Utilizing the centromere B fusion protein (CENP-B) and specific, matched monoclonal antiisotype reagents in an enzyme-linked immunosorbent assay, we found that anti-CENP-B autoantibodies were skewed to the IgG1 isotype. The overall kappa:lambda light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain-skewed sera showed polyclonal patterns. Our findings are consistent with the anti-CENP-B autoantibody response being a chronic, antigen-driven response.

Cite this paper

@article{Eisenberg1989IsotypeAO, title={Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality.}, author={Robert Eisenberg and William C Earnshaw and B J Bordwell and Stephen Craven and Robert L Cheek and Naomi F. Rothfield}, journal={Arthritis and rheumatism}, year={1989}, volume={32 10}, pages={1315-8} }