Isotopic labeling of DNA in rat adipose tissue: evidence for proliferating cells associated with mature adipocytes.

@article{Klyde1979IsotopicLO,
  title={Isotopic labeling of DNA in rat adipose tissue: evidence for proliferating cells associated with mature adipocytes.},
  author={B J Klyde and Jules Hirsch},
  journal={Journal of lipid research},
  year={1979},
  volume={20 6},
  pages={691-704}
}
The intraperitoneal administration of [3H]thymidine to adult rats resulted in the rapid appearance of label in the adipocyte fraction of collagenase digests of adipose tissue. Low-speed centrifugation followed by freezing and slicing showed the label to be uniformly distributed in the adipocyte fraction. The presence of label in DNA was confirmed by hydrolysis with deoxyribonuclease and by inhibition of incorporation with hydroxyurea. Organelle fractionation revealed that the label was… CONTINUE READING

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