Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase.

  title={Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase.},
  author={B N Leichus and John S Blanchard},
  volume={33 48},
Glycerol dehydrogenase catalyzes the reversible NAD(+)-dependent oxidation of glycerol to form dihydroxyacetone. Initial velocity, product, and dead-end inhibition studies performed for the forward and reverse reactions support an ordered kinetic mechanism with NAD+ binding first and NADH released last. A monovalent cation is required for enzymatic activity and glycerol binding, with K+ having the highest activity as measured by V. The pH dependence of the kinetic parameters V and V/Kglycerol… CONTINUE READING