Isotope labeling and microautoradiography of active heterotrophic bacteria on the basis of assimilation of 14CO(2).

Abstract

Most heterotrophic bacteria assimilate CO(2) in various carboxylation reactions during biosynthesis. In this study, assimilation of (14)CO(2) by heterotrophic bacteria was used for isotope labeling of active microorganisms in pure cultures and environmental samples. Labeled cells were visualized by microautoradiography (MAR) combined with fluorescence in… (More)

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