Isothermal Titration Calorimetric Study of RNase-A Kinetics (cCMP → 3'-CMP) Involving End-Product Inhibition

Purpose. Isothermal titration calorimetry (ITC) and progress curve analysis was used to measure the enzyme kinetic parameters (K M and k cat) of the hydrolysis of cCMP by RNase-A, a reaction that includes end-product competitive inhibition by 3'-CMP. Methods. The heat generated from injection of 9-15 μl cCMP (20 mM) into bovine pancreatic RNase-A (600 nM… CONTINUE READING