Isothermal DNA amplification coupled with DNA nanosphere-based colorimetric detection.

@article{Tan2005IsothermalDA,
  title={Isothermal DNA amplification coupled with DNA nanosphere-based colorimetric detection.},
  author={Eric Tan and Jennifer C. Y. Wong and Doris Nguyen and Yolanda Zhang and Barbara Erwin and Lori K Van Ness and Shenda M. Baker and David J. Galas and Angelika Niemz},
  journal={Analytical chemistry},
  year={2005},
  volume={77 24},
  pages={7984-92}
}
We present a simple, rapid method for detecting short DNA sequences that combines a novel isothermal amplification method (EXPAR) with visual, colorimetric readout based on aggregation of DNA-functionalized gold nanospheres. The reaction is initiated by a trigger oligonucleotide, synthetic in nature for this proof-of-principle study, which is exponentially amplified at 55 degrees C and converted to a universal reporter oligonucleotide capable of bridging two sets of DNA-functionalized gold… CONTINUE READING