Isomerization of Asp-Asp motif in model peptides and a monoclonal antibody Fab fragment.

@article{Yi2013IsomerizationOA,
  title={Isomerization of Asp-Asp motif in model peptides and a monoclonal antibody Fab fragment.},
  author={Li Yi and Nia S Beckley and Benson Gikanga and Jennifer E. Zhang and Y. John Wang and Hung-Wei Chih and Vikas Kumar Sharma},
  journal={Journal of pharmaceutical sciences},
  year={2013},
  volume={102 3},
  pages={947-59}
}
Isomerization of aspartyl (Asp or D) residues is a critical degradation route to consider for stable monoclonal antibody formulations. Among the known hotspot sequences, the DD motif is relatively understudied. To gain mechanistic insights, we used model hexapeptides, YADXFK, YADDXK, and DIDDDM, as surrogates for the hotspots in a Fab protein (YADDFK and… CONTINUE READING